Molecular Mechanisms of KIT Receptor Dimerization and Oncogenic Activation Revealed by Multiscale Simulations

Citation (APA 7)

Chiariello, M. G., Biswas, A. D., Gratteri, C., Guarnetti Prandi, I., Marrink, S. J., Beccari, A., & Talarico, C. (2026). Molecular Mechanisms of KIT Receptor Dimerization and Oncogenic Activation Revealed by Multiscale Simulations.

Abstract

The receptor tyrosine kinase KIT is essential for cell proliferation and differentiation, and its aberrant activation contributes to cancers such as acute myeloid leukemia and gastrointestinal stromal tumors. KIT activation normally requires stem cell factor (SCF)-induced dimerization, whereas oncogenic mutations can drive ligand-independent signalling. Here, we employ multiscale molecular dynamics simulations based on the Martini 3 coarse-grained (CG) force field to investigate the dimerization mechanisms of full-length KIT in both wild-type (WT) and oncogenic mutant forms within a realistic membrane environment. For the WT receptor, simulations capture SCF-driven dimerization through a cooperative “zipper-like” mechanism involving sequential domain-domain interactions. In contrast, the T417I, Δ418–419 mutant forms stable dimers spontaneously in the absence of SCF, stabilized by enhanced D5–D5 hydrophobic contacts. Back-mapping and atomistic refinement yielded the first all-atom (AA) structural model of the full-length KIT(T417I, Δ418–419) mutant, consistent with the V-shaped extracellular conformation observed experimentally. This study provides mechanistic insight into KIT activation and establishes a Martini 3 based framework for simulating receptor assembly and guiding the design of antibodies or small molecules that disrupt aberrant dimerization.